( A 2 or B 22), the diffusion interaction parameter ( k D), turbidity or opalescence and the temperature of protein melting or aggregation onset ( T m, T onset). DYNAMICS can control the NanoStar and acquire data directly, or read in DYNAMICS Touch data files.ĭYNAMICS is offered with a 21 CFR Part 11 compliant Security Pack option, enabling the use of the DynaPro NanoStar in a GMP environment for quality control or at-line process monitoring.Ĭoupled with the versatile and user-friendly DYNAMICS software and DYNAMICS Touch on-board app, the NanoStar may be utilized to measure not just size, size distributions, particle concentration and weight-average molar mass, but also solution properties relevant to sample stability: the second virial coefficient It performs advanced analyses and compares multiple data files to identify trends and statistics. You can even send the report to your phone! Full-featured analyses with DYNAMICS ® DYNAMICS is our powerful PC-based, comprehensive software package for collecting, analyzing, and reporting batch dynamic and static light scattering data. Data files and concise PDF reports can be stored on the instrument, transferred via the network or downloaded to a flash drive. It tells you if the data quality is sufficient for confidence in the results, and displays them instantly. Via the full-size touch screen display, DYNAMICS Touch guides you through the entire setup and measurement process. DYNAMICS Touch is an intuitive-yet capable-DLS measurement app that resides right on the NanoStar. No need to boot up your lab PC, launch the DLS software, connect to the instrument and worry about where your data files are stored. The NanoStar is the only DLS instrument to provide a fully integrated, walk-up measurement experience. Walk-up operation with DYNAMICS ® Touch ™ To accommodate scarce and precious samples, specially designed quartz cuvettes afford robust, accurate measurements with as little as 2 µL of solution, while our proprietary disposable cuvettes provide comparable sensitivity with as little as 4 µL! The DynaPro ® NanoStar ® II is a unique cuvette-based DLS instrument used for the analysis of proteins, gene therapy vectors, viruses and virus-like particles, promiscuous inhibitors, micelles, liposomes, lipid and metallic nanoparticles, and more. Traditional dynamic light scattering, just better. Dynamic & Electrophoretic Light Scattering.hard sphere, globular, dendrimer, chain stiffness, and degree of branching). Hydrodynamic sizes are more easily measured than radii of gyration and can be measured over a wider range of sizes. The conversion from hydrodynamic radius to radius of gyration is a function of chain architecture (including questions of random coil vs. The hydrodynamic radius is not the same as the radius of gyration. Radius calculations are the same except for a factor of two.Īlso, a note to those interested in polymer size. That is, the determined particle size is the size of a sphere that diffuses the way as your particle.įor those who work with protein sizing and other areas where hydrodynamic radius is more commonly used, note that the development here is around diameter. Finally, and most importantly, it reminds the analyst that the particle size determined by dynamic light scattering is the hydrodynamic size. Temperature is even more important due to the viscosity term since viscosity is a stiff function of temperature. The first is that sample temperature is important, at it appears directly in the equation. However, the equation does serve as important reminder about a few points. The calculations are handled by instrument software. T is thermodynamic temperature (we control this).k B is Boltzmann’s constant (we know this).D t is the translational diffusion coefficient (we find this by dynamic light scattering).D h is the hydrodynamic diameter (this is the goal: particle size!).
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